P01.47. Analgesic and antiedematogenic effect of Energycare™ in the mice model of ischemia-reperfusion of the paw: involvement of anti-inflammatory cytokines

Methods Male Swiss mice were used in the experiments. All procedures were approved by the University Animal Ethics Comittee (CEUA/Unisul protocol n# 11.022.4.08.IV). For induction of IR an elastic ring (1.2mm diameter) was positioned in the right ankle of anesthetized mice (chloral hydrate 7%, 0.6ml/kg, ip). After three hours the ring was cut allowing reperfusion. Experimental groups were: Sham (anesthetized but not subjected to IR), IR, Sham + EC, and IR + EC. For treatment, EC “Dream Pillow” or “Sham Pillow” was placed in the bottom of the animals boxes on the 3rd removed on the 10th and replaced on the 11th postoperative (PO) day. Mechanical hypersensitivity was determined as response frequency to 10 presentations of a 0.4g von Frey filament. Paw volume was assessed with a plethysmometer. Interleukin (IL)-10 and IL-1ra levels (paw skin and intraplantar muscles) were determined by enzyme-linked immunosorbent assay on the fourth day PO after 24h exposure to EC.